Biacore X

Description

Biacore X (GE Healthcare) is manual surface plasmon resonance based refractometer, appropriate for the introductory investigation of interactions with unknown properties. We recommend to use Biacore X for the complex interactions and initial screenings. The chip with two flow cells is being used and all the reagents are injected manually. For the immobilization via amine group the sensor chip CM5 is used (Product number BR-1000-12).

Specification

Sample load manual
Size limit > 180 Da
Flow rate 1-100 μl/min
Typical concentrations (proteins) pM - μM
Sample volume 40-90 μl
Refractive index 1,33 - 1,40
Analysis temperature 4°C - 40 °C
Flow cell 2
Reference subtraction yes

Contact

Gregor Bajc

gregor.bajc@bf.uni-lj.si

Biotehniška fakulteta

Večna pot 111, 1000 Ljubljana

Docs

  • Specification
  • Biacore X-Getting Started
  • BiaEvaluation handbook