Biacore X100
Description
Biacore X100 (GE Healthcare) is automated surface plasmon resonance based refractometer. It can be used for the analysis of 15 different analytes in single run. The chip with two flow cells are compatible with Biacore X. The unit has incorporated degasser and offers single-cycle titration and calibration-free concentration analysis.
Specification
| Sample load | automatic |
| Size limit | > 100 Da |
| Flow rate | 1-100 μl/min |
| Typical concentrations (proteins) | pM - μM |
| Sample volume | 50-120 μl |
| Refractive index | 1,33 - 1,40 |
| Analysis temperature | 4°C - 45°C |
| Flow cell | 2 |
| Reference subtraction | yes |
| different buffer conditions | yes |
| Sample concentration | 10e-3 - 10e-11 M |
| Association constant (ka) | 10e3 - 10e7 /Ms |
| Dissociation constant (kd) | 10e-5 - 0.5 /s |
| Baseline noise | <0.1 RU |
| Baseline drift | <0.3 RU/min |
| Calibration-free concentration analysis | yes |
| Single-cycle titration | yes |