MicroCal VP-ITC
Description
The MicroCal VP-ITC is isothermal titration calorimeter that offers the ability to investigate any biomolecular interactions. It uses the Isothermal Titration Calorimetry technique to measure the binding affinity and thermodynamic parameters involved in a variety of molecular interactions. It is fast, convenient, label free and in-solution technique which delivers the information needed to characterize the molecular interactions of proteins, antibodies, nucleic acids and other biomolecules. The system is widely used in research environments for lead optimization, hit validation, assessing the driving mechanisms of binding and studying enzyme kinetics and inhibitor design.
The equipment is accessible to both internal and external users upon prior request. Individuals interested in utilizing the equipment should contact the device administrator. Only trained operators can schedule the use of the equipment, or alternatively, the measurement can be conducted by the experienced staff of the Department of Molecular Biology and Nanobiotechnology.
Specifications
| Measurement type | Affinity (KD), enthalpy (∆H), entropy (∆S), stoichiometry |
| Sample volume | 2 ml |
| Cell volume | 1400 µL |
| Injection syringe volume | 300 µL |
| Sample capacity | 4-8 samples per 8 h |
| Noise | 0.5 ncal/s |
| Temperature range | 2-80° C |
| Response time | 20 s |
Sample requirements
| Purity/quality of the sample needed | Pure samples are required and concentrations determined as accurately as possible (spectrophotometrically). |
| Amount needed for a typical measurement | 400 μl of the titrant (syringe) with a concentration approx. 20-fold higher than anticipated KD and 1.8 ml of the macromolecule (sample cell) with a concentration 10-15 × lower than the macromolecule concentration. |
| Buffer considerations |
The two binding partners should be in identical buffers. DTT and unstable chemicals should not be used. DMSO should be matched extremely well between the cell and the syringe. |
| Other requirements |
Guest researchers are kindly asked to fill in the Sample request form regarding sample quality, experimental conditions and plausible previous results. Buffers and consumables for experiments can be provided by the National Institute of Chemistry by prior arrangement. |
Contact
Kristina ElerÅ¡iÄ FilipiÄ
kristina.elersic.filipic@ki.si
Kemijski inštitut
Hajdrihova ulica 19, 1000 Ljubljana
Documents
Kristina ElerÅ¡iÄ FilipiÄ
kristina.elersic.filipic@ki.si
Kemijski inštitut
Hajdrihova ulica 19, 1000 Ljubljana