MicroCal VP-ITC

Opis

Izotermalni titracijski kalorimeter VP-ITC (MicroCal) omogoča proučevanje molekulskih interakcij med proteini, protitelesi, nukleinskimi kislinami in drugimi molekulami. Uporablja se za optimizacijo in overitev tarčnih spojin, za načrtovanje inhibitorjev, študijo encimske kinetike ... Meri tako afiniteto kot tudi termodinamske lastnosti interakcije.

Lastnosti

Meritve afiniteta (KD), entalpija (∆H), entropija (∆S), stehiometrija 
Volumen vzorca 2 ml
Volumen celice 1400 µL
Volumen brizge  300 µL
Sposobnost   4-8 vzorcev v 8 h
 Šum  0,5 ncal/s
 Temp. območje  2-80° C
 Odzivni čas  20 s

Priprava vzorcev

Purity/quality of the sample needed Pure samples are required and concentrations determined as accurately as possible (spectrophotometrically).
Amount needed for a typical measurement 400 μl of the titrant (syringe) with a concentration approx. 20-fold higher than anticipated KD and 1.8 ml of the macromolecule (sample cell) with a concentration 10-15 × lower than the macromolecule concentration.
Buffer considerations

The two binding partners should be in identical buffers.

DTT and unstable chemicals should not be used.

DMSO should be matched extremely well between the cell and the syringe.

Other requirements

Guest researchers are kindly asked to fill in the Sample request form regarding sample quality, experimental conditions and plausible previous results.

Buffers and consumables for experiments can be provided by the National Institute of Chemistry by prior arrangement.

Kontakt

Neža Omersa

neza.omersa@ki.si

Kemijski inštitut

Hajdrihova ulica 19, 1000 Ljubljana

Dokumenti

  • Lastnosti
  • MicroCal VP-ITC Data sheet
  • Single Injection Method software for MicroCal VP-ITC system