MicroCal VP-ITC
Opis
Izotermalni titracijski kalorimeter VP-ITC (MicroCal) omogoča proučevanje molekulskih interakcij med proteini, protitelesi, nukleinskimi kislinami in drugimi molekulami. Uporablja se za optimizacijo in overitev tarčnih spojin, za načrtovanje inhibitorjev, študijo encimske kinetike ... Meri tako afiniteto kot tudi termodinamske lastnosti interakcije.
Oprema je na voljo notranjim in zunanjim uporabnikom ob predhodem povpraševanju.
Zainteresirani uporabniki naj kontaktirajo skrbnico naprave.
Opremo lahko ob vnaprejšnjem dogovoru uporabljajo usposobljeni operaterji ali pa meritev izvede osebje Odseka za molekularno biologijo in nanobiotehnologijo.
Lastnosti
Meritve | afiniteta (KD), entalpija (∆H), entropija (∆S), stehiometrija |
Volumen vzorca | 2 ml |
Volumen celice | 1400 µL |
Volumen brizge | 300 µL |
Sposobnost | 4-8 vzorcev v 8 h |
Šum | 0,5 ncal/s |
Temp. območje | 2-80° C |
Odzivni čas | 20 s |
Priprava vzorcev
Purity/quality of the sample needed | Pure samples are required and concentrations determined as accurately as possible (spectrophotometrically). |
Amount needed for a typical measurement | 400 μl of the titrant (syringe) with a concentration approx. 20-fold higher than anticipated KD and 1.8 ml of the macromolecule (sample cell) with a concentration 10-15 × lower than the macromolecule concentration. |
Buffer considerations |
The two binding partners should be in identical buffers. DTT and unstable chemicals should not be used. DMSO should be matched extremely well between the cell and the syringe. |
Other requirements |
Guest researchers are kindly asked to fill in the Sample request form regarding sample quality, experimental conditions and plausible previous results. Buffers and consumables for experiments can be provided by the National Institute of Chemistry by prior arrangement. |
Kontakt
Kristina Eleršič Filipič
kristina.elersic.filipic@ki.si
Kemijski inštitut
Hajdrihova ulica 19, 1000 Ljubljana
Dokumenti
Kristina Eleršič Filipič
kristina.elersic.filipic@ki.si
Kemijski inštitut
Hajdrihova ulica 19, 1000 Ljubljana